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Antibodies certain to the blocking peptide not bind to the epitope to the goal protein. By evaluating the staining from your blocked antibody versus the antibody alone, you can see which staining is distinct.
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This information outlines further doable optimization of mobile and immunoassay parameters to make certain the very best outcomes are...
This tutorial outlines further more achievable optimization of cellular and immunoassay parameters to be sure the very best success are...
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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Blocking peptides are peptides that bind exclusively on the focus on antibody and block antibody binding. These peptide generally consists of the epitope identified through the antibody.
Concentrate on Information and facts Cyclin E belongs to the highly conserved cyclin family, whose users are characterised by a remarkable periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation click here styles which lead to your temporal coordination of every mitotic event. Cyclin E forms a fancy with and functions to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells progress as a result of S stage.
Overexpression of this gene has long been noticed in many tumors, which leads to chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was found to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant part in promoting cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two further splice variants ended up noted but specific nucleotide sequence information is just not nonetheless obtainable.
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